Extraction of biogenic amines using sorbent materials containing immobilized crown ethers
Mardiana Saaid1, Bahruddin Saad, Ismail Ab Rahman
1School of Chemical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
Talanta
|December 17, 2009
Summary
New sorbent materials based on crown ether ligands were developed for selective extraction of spermidine (SPD). The AB18C6-MS sorbent demonstrated high capacity and selectivity for SPD in food samples, enabling efficient separation for analysis.
Area of Science:
- Analytical Chemistry
- Materials Science
- Separation Science
Background:
- Biogenic amines are important compounds in food and biological systems.
- Selective extraction methods are crucial for their accurate analysis.
- Crown ether-based materials offer potential for specific molecular recognition.
Purpose of the Study:
- To prepare and characterize novel mesoporous silica-supported crown ether sorbents.
- To evaluate their efficiency in extracting biogenic amines.
- To develop a selective method for spermidine (SPD) extraction from food samples.
Main Methods:
- Chemical immobilization of crown ether ligands (1-aza-18-crown-6, 1,4,10,13-tetraoxa-7,16-diazacyclo octadecane, 4'-aminobenzo-18-crown-6) onto mesoporous silica.
- Characterization using FT-IR, SEM-EDX, elemental analysis, and N2 adsorption-desorption.
- Batch sorption experiments to optimize pH, concentration, and contact time.
- Reversed-phase high-performance liquid chromatography (RP-HPLC) for separation and analysis.
Main Results:
- Three sorbent materials (A18C6-MS, DA18C6-MS, AB18C6-MS) were successfully synthesized and characterized.
- All sorbents showed high selectivity for spermidine (SPD) over other tested biogenic amines.
- AB18C6-MS exhibited the highest extraction capacity and selectivity for SPD.
- The AB18C6-MS sorbent demonstrated good reusability for at least three cycles with >85% extraction efficiency.
- The optimized method was successfully applied to separate SPD in real food samples.
Conclusions:
- Mesoporous silica-supported crown ether sorbents are effective for biogenic amine extraction.
- AB18C6-MS is a highly selective and reusable material for spermidine (SPD) isolation.
- The developed method provides a valuable tool for SPD determination in food matrices prior to HPLC analysis.
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