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Updated: Jun 17, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Fabrication and evaluation of chiral monolithic column modified by beta-cyclodextrin derivatives
Yingjie Li1, Chunhui Song, Lingyi Zhang
1Key Laboratory of Functional Inorganic Material Chemistry, Ministry of Education of the People's Republic of China, Heilongjiang University, Xuefu Road 74, Harbin 150080, Nangang District, Heilongjiang, PR China.
Abstract:
Based on combination of chiral recognition ability of beta-cyclodextrin (beta-CD) derivatives and flexibility of monolithic material, a series of chiral stationary phases (CSPs) were prepared by the immobilization of beta-CD and three of its derivatives to the epoxy-activated poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith under mild condition. Immobilization condition for the connection reaction by different functional groups and bonding ways was studied to obtain good enantiomer selectivity. Prepared CSPs were evaluated by separating racemic mixtures of eight amino acids and two chiral drugs with capillary electrochromatography (CEC).
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