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Updated: Jun 17, 2026

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

A rapid multiplex assay for nucleic acid-based diagnostics.

Alina Deshpande¹, Jason Gans, Steven W Graves

¹Decision Applications Division, Mail Stop K551, Los Alamos National Laboratory, Los Alamos, NM 87545, United States. deshpande_a@lanl.gov

Journal of Microbiological Methods

|December 17, 2009

Summary

This summary is machine-generated.

We created a fast, single-tube multiplex oligonucleotide ligation-PCR (MOL-PCR) assay for detecting multiple nucleic acid targets simultaneously. This assay shows potential for rapid diagnostics and surveillance of biothreat agents.

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Visual Detection of Multiple Nucleic Acids in a Capillary Array

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Published on: November 15, 2017

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Published on: March 13, 2018

Area of Science:

Molecular Biology
Biotechnology
Diagnostic Assays

Background:

Multiplex nucleic acid detection is crucial for diagnostics and surveillance.
Existing ligation-based assays can be complex and time-consuming.
There is a need for rapid, robust, and simplified multiplex assays.

Purpose of the Study:

To develop a rapid, single-tube, multiplex nucleic acid assay.
To adapt the assay to a microsphere array detection platform.
To demonstrate the assay's utility for detecting biothreat agents.

Main Methods:

Developed multiplex oligonucleotide ligation-PCR (MOL-PCR) assay.
Utilized a single-tube reaction followed by Luminex microsphere array detection.
Designed modular detection probes for target detection, amplification, and capture.

Main Results:

Achieved rapid detection (under 4 hours) of diverse nucleic acid signatures.
Successfully demonstrated simultaneous detection of unique sequences and single nucleotide polymorphisms.
Applied the assay to detect three biothreat agents: B. anthracis, Y. pestis, and F. tularensis.

Conclusions:

The multiplex oligonucleotide ligation-PCR (MOL-PCR) assay is rapid, robust, and simplifies multiplex nucleic acid detection.
The assay's modular design and microsphere platform enable versatile applications.
This assay holds significant potential for use in diagnostics and surveillance, particularly for biothreat agents.