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Updated: Jun 17, 2026
Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice
Published on: January 6, 2018
A specific subset of mouse bone marrow cells has smooth muscle cell differentiation capacity-brief report
Pat Metharom1, Arun H S Kumar, Sharon Weiss
1Centre for Research in Vascular Biology, BioSciences Institute, University College Cork, College Road, Cork City, Ireland.
Objective:
To determine whether CX(3)CR1(+) bone marrow cells have the capacity for smooth muscle cell (SMC) differentiation.
Methods And Results:
CX(3)CR1(+) and CX(3)CR1(-) cells were isolated from marrow of CX(3)CR1 transgenic mice and cultured in SMC differentiation media. Phenotypic and functional analyses showed only CX(3)CR1(+) bone marrow cells exhibit colony cell outgrowth with SMC-specific protein expression, calcium signaling, and contraction responses similar to mature SMC.
Conclusions:
CX(3)CR1 marks a bone marrow cells population that enriches for progenitors with capacity to differentiate in vitro into SMC-like cells.
