Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Dec 23, 2025

Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis

Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis

Published on: May 9, 2025

Palifermin induces alveolar maintenance programs in emphysematous mice.

Ali O Yildirim¹, Vandana Muyal, Gerrit John

¹Clinical Research Group Chronic Airway Diseases, Medical Faculty, Philipps-University Marburg, Marburg, Germany.

American Journal of Respiratory and Critical Care Medicine

|December 17, 2009

Summary

Therapeutic application of DeltaN23-KGF shows potential in emphysema treatment by promoting alveolar regeneration. This involves stimulating cell proliferation and releasing TGF-beta1, suggesting a novel approach for lung repair.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Transcriptomic Atlas of Chronic Lung Allograft Dysfunction.

The European respiratory journal·2026

Same author

Sexual health following hyperbaric oxygen treatment in pelvic cancer survivors with radiation injuries: a longitudinal observational study.

The journal of sexual medicine·2026

Same author

Longitudinal clinical proteomics reveals pneumonia type-specific protein biomarkers and autoantibodies.

JCI insight·2026

Same author

Relevance of clinical ethics support services in specialized outpatient palliative care teams and inpatient hospices.

BMC palliative care·2026

Same author

Nuclear protein 1 is a cell death regulator in primary human airway epithelial cells and reduced in idiopathic pulmonary fibrosis.

Scientific reports·2026

Same author

Longitudinal clinical proteomics reveals pneumonia type-specific protein biomarkers and autoantibodies.

medRxiv : the preprint server for health sciences·2026

Area of Science:

Pulmonary Medicine
Regenerative Medicine
Cell Biology

Background:

Emphysema involves alveolar destruction and airspace enlargement, posing a significant challenge for therapeutic regeneration.
Current emphysema therapies struggle to induce alveolar regeneration.

Purpose of the Study:

To investigate the potential of palifermin (DeltaN23-KGF) in inducing lung tissue regeneration in an emphysema model.
To explore the mechanisms underlying DeltaN23-KGF-mediated regenerative effects.

Main Methods:

Pulmonary emphysema was induced in mice using porcine pancreatic elastase.
Mice received therapeutic oropharyngeal aspiration of DeltaN23-KGF.
Regenerative responses were assessed using plethysmography, stereology, PCR, Western blotting, and immunohistochemistry.

More Related Videos

Alveolar Macrophage Phagocytosis and Bacteria Clearance in Mice

Alveolar Macrophage Phagocytosis and Bacteria Clearance in Mice

Published on: March 2, 2019

Phenotyping Mouse Pulmonary Function In Vivo with the Lung Diffusing Capacity

Phenotyping Mouse Pulmonary Function In Vivo with the Lung Diffusing Capacity

Published on: January 6, 2015

Related Experiment Videos

Last Updated: Dec 23, 2025

Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis

Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis

Published on: May 9, 2025

Alveolar Macrophage Phagocytosis and Bacteria Clearance in Mice

Alveolar Macrophage Phagocytosis and Bacteria Clearance in Mice

Published on: March 2, 2019

Phenotyping Mouse Pulmonary Function In Vivo with the Lung Diffusing Capacity

Phenotyping Mouse Pulmonary Function In Vivo with the Lung Diffusing Capacity

Published on: January 6, 2015

Main Results:

DeltaN23-KGF treatment largely reversed airflow limitation and partially reversed airspace enlargement in emphysematous mice.
It stimulated proliferation of lung epithelium, endothelium, and fibroblasts.
The treatment increased expression of growth factors like VEGF and TGF-beta1, crucial for tissue repair and elastin production.

Conclusions:

Therapeutic DeltaN23-KGF can initiate alveolar maintenance programs in emphysematous lungs.
The observed regenerative effects on lung interstitial tissue are linked to TGF-beta1 derived from alveolar epithelial type 2 cells.