Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Diabetic Retinopathy01:27

Diabetic Retinopathy

DefinitionDiabetic retinopathy is a microvascular complication of diabetes affecting the retinal blood vessels.Risk FactorsDiabetic retinopathy is present in almost all individuals with type 1 diabetes and more than 60% of those with type 2 diabetes after two decades of disease.The risk increases with poor glycemic control, hypertension, dyslipidemia, smoking, pregnancy, and puberty.Although cataracts and glaucoma are also more frequent in people with diabetes, retinopathy remains the leading...
Type II Diabetes I: Introduction01:26

Type II Diabetes I: Introduction

Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterized by insulin resistance, in which target tissues such as the liver, muscle, and adipose tissue respond poorly to insulin. It is also associated with inadequate compensatory insulin secretion, where pancreatic β-cells fail to produce sufficient insulin. Together, these abnormalities lead to persistent hyperglycemia.EtiologyT2DM develops through a complex interaction of genetic predisposition and environmental or...
Type II Diabetes II: Pathophysiology01:24

Type II Diabetes II: Pathophysiology

PathophysiologyType 2 diabetes mellitus (T2DM ) is a chronic metabolic disorder characterized by insulin resistance and progressive pancreatic β-cell dysfunction, leading to impaired glucose homeostasis. It results from interactions among genetic predisposition, environmental factors, and metabolic stressors, such as overnutrition and a sedentary lifestyle.Insulin Resistance and Glucose DysregulationEarly T2DM involves insulin resistance in skeletal muscle, adipose tissue, and the liver.
Type I Diabetes II: Pathophysiology01:26

Type I Diabetes II: Pathophysiology

Type 1 diabetes mellitus arises from an immune-mediated destruction of pancreatic β-cells, resulting in an absolute deficiency of insulin. This process develops in genetically susceptible individuals when autoimmunity, environmental exposures, and immunologic dysregulation converge to trigger a targeted attack on the insulin-producing cells of the pancreas. The β-cells are located within the islets of Langerhans and are essential for regulating blood glucose by facilitating cellular uptake of...
Diabetic Neuropathy01:22

Diabetic Neuropathy

DefinitionDiabetic neuropathy is nerve damage caused by long-standing diabetes mellitus. It results directly from prolonged high blood sugar levels.PathophysiologyThe pathophysiology of diabetic neuropathy involves both metabolic and vascular disturbances triggered by chronic hyperglycemia.Metabolic injury: Elevated glucose levels activate the polyol pathway within nerve cells, leading to the accumulation of sorbitol and fructose. This increases oxidative stress, disrupts normal nerve...
Pathophysiology of Diabetes01:20

Pathophysiology of Diabetes

Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia. The four categories of diabetes are type 1 diabetes, type 2 diabetes, other specific types of diabetes, and gestational diabetes.
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility, suggesting a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multi-sensor tracking reveals the extent of nocturnal migration in landbirds.

Current biology : CB·2026
Same author

Recommendations and considerations for hydroxyl radical protein footprinting-mass spectrometry.

Nature methods·2026
Same author

scProfiterole: Clustering of Single-Cell Proteomic Data Using Graph Contrastive Learning via Spectral Filters.

bioRxiv : the preprint server for biology·2026
Same author

What Does Next-Generation Mass Spectrometry Offer for Proteomics? A Comprehensive Platform Comparison.

Journal of proteome research·2026
Same author

Hydroxyl radical footprinting modification reveals an intradomain communication pathway in EFL1 disrupted by a Shwachman-Diamond syndrome-associated mutation.

Protein science : a publication of the Protein Society·2026
Same author

Proteomics and Bioinformatics Profiles of Human Mesothelial Cell Line MeT-5A.

Proteomes·2026

Related Experiment Video

Updated: Jun 17, 2026

Mouse Electroacupuncture Fixation Device Fabrication for Electroacupuncture Pretreatment in Diabetic Cardiomyopathy Mouse Model
05:58

Mouse Electroacupuncture Fixation Device Fabrication for Electroacupuncture Pretreatment in Diabetic Cardiomyopathy Mouse Model

Published on: April 18, 2025

Molecular targets for diabetes mellitus-associated erectile dysfunction.

Elizabeth Yohannes1, Jinsook Chang, Moses T Tar

  • 1Center for Proteomics and Bioinformatics, Case Western Reserve University, Cleveland, Ohio 44106, USA.

Molecular & Cellular Proteomics : MCP
|December 17, 2009
PubMed
Summary

This study investigated protein changes in rat erectile tissue due to diabetes. Key proteins involved in collagen formation, apoptosis, and lipid metabolism were identified, offering insights into diabetic erectile dysfunction.

Related Experiment Videos

Last Updated: Jun 17, 2026

Mouse Electroacupuncture Fixation Device Fabrication for Electroacupuncture Pretreatment in Diabetic Cardiomyopathy Mouse Model
05:58

Mouse Electroacupuncture Fixation Device Fabrication for Electroacupuncture Pretreatment in Diabetic Cardiomyopathy Mouse Model

Published on: April 18, 2025

Area of Science:

  • Proteomics
  • Molecular Biology
  • Endocrinology

Background:

  • Diabetes mellitus is a complex metabolic disorder with significant vascular and neurological complications.
  • Erectile dysfunction (ED) is a common comorbidity of diabetes, impacting quality of life.
  • Understanding the molecular mechanisms underlying diabetic ED is crucial for developing targeted therapies.

Purpose of the Study:

  • To compare protein expression profiles in rat corporal smooth muscle tissue between streptozotocin-induced diabetes mellitus (STZ-DM) and age-matched controls (AMCs).
  • To identify novel candidate proteins involved in the pathogenesis of ED in the context of diabetes.
  • To elucidate the temporal changes in protein expression at early (1 week) and later (2 months) stages of hyperglycemia.

Main Methods:

  • Quantitative proteomics using two-dimensional gel electrophoresis and mass spectrometry.
  • Analysis of protein expression changes in STZ-DM versus AMC rat corpora tissues at two time points.
  • Western blot validation of key identified proteins (p53, 14-3-3-gamma, Hsp47).

Main Results:

  • Differential expression of 170 protein spots, identifying 57 unique proteins.
  • Down-regulation of collagen precursors (type 1) and Hsp47 (collagen folding mediator).
  • Up-regulation of proteins involved in apoptosis (p53, 14-3-3-gamma) and lipid metabolism (apoA-I, apoA-IV).
  • Western blot confirmed decreased Hsp47 and increased p53 and 14-3-3-gamma in diabetic rats.

Conclusions:

  • Identified novel candidate proteins associated with ED in STZ-induced diabetes.
  • Protein expression alterations suggest impaired collagen synthesis, increased apoptosis, and altered lipid metabolism contribute to diabetic ED.
  • Hsp47 decrease and p53/14-3-3-gamma increase are significant molecular changes in diabetic erectile dysfunction.