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A nucleolin-targeted multimodal nanoparticle imaging probe for tracking cancer cells using an aptamer
Do Won Hwang1, Hae Young Ko, Jung Hwan Lee
1Program in Neuroscience, Seoul National University, Seoul, Korea.
Summary
This study introduces a novel multimodal nanoparticle probe for cancer imaging. The AS1411 aptamer-targeted probe enables concurrent fluorescence, radionuclide, and MRI detection of cancer cells in vivo.
Area of Science:
- Nanotechnology
- Molecular Imaging
- Biomedical Engineering
Background:
- Molecular imaging advances enable noninvasive tracking of cancer cells.
- Nanoparticle probes offer potential for enhanced in vivo cancer detection.
Purpose of the Study:
- To develop a multimodal cancer-targeted imaging system.
- To utilize nanoparticle probes for concurrent fluorescence, radionuclide, and MRI in vivo.
Main Methods:
- Synthesized a cobalt-ferrite nanoparticle (MF) with AS1411 aptamer targeting nucleolin.
- Labeled the nanoparticle with Gallium-67 for radionuclide imaging and assessed via TEM, fluorescence microscopy, and MRI.
- Evaluated in vivo targeting specificity and imaging capabilities in a cancer cell model.
Main Results:
- The MFR-AS1411 nanoparticle demonstrated spherical morphology and good dispersion.
- Specific fluorescence and radionuclide signals were observed in nucleolin-expressing cancer cells.
- In vivo studies confirmed MFR-AS1411 specifically targeted tumors, showing distinct MRI signals.
Conclusions:
- Developed a nanoparticle-based imaging probe using the AS1411 aptamer for cancer-specific detection.
- The multimodal imaging strategy offers a versatile tool for in vivo cancer diagnosis.
