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Updated: Jun 17, 2026
08:23
Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
Plasma nitrite response in older women to a physical function test.
Devon A Dobrosielski1, Tennille Presley, Andreas Perlegas
1Department of Health and Exercise Science, Wake Forest University, Winston-Salem, NC, USA. ddobros1@jhmi.edu
Aging Clinical and Experimental Research
|December 17, 2009
Summary
A 400-meter walk test reduced plasma nitrite levels in older women, indicating a potential impact on vascular function and overall health. This finding highlights the physiological response to physical activity in this age group.
Area of Science:
- Gerontology
- Cardiovascular Physiology
- Exercise Science
Background:
- Nitric oxide (NO) is crucial for blood flow to muscles, impacting functional health in older adults.
- Plasma nitrite serves as a key indicator of nitric oxide (NO) bioavailability.
- Physical function tests may influence NO levels, affecting vascular health in the elderly.
Purpose of the Study:
- To investigate the impact of a standard physical function test on plasma nitrite concentrations.
- To assess changes in vascular nitric oxide (NO) bioavailability following a 400-meter walk test in older adults.
Main Methods:
- Blood samples were collected from participants before, immediately after, and 10 minutes post-walk.
- Plasma nitrite levels were measured using chemiluminescence analysis.
- The study involved 26 women aged 79±4 years.
Main Results:
- Plasma nitrite concentrations decreased by approximately 22% after the 400-meter walk.
- A significant inverse relationship was observed between walking speed and the percent change in plasma nitrite (r=-0.550, p=0.004).
Conclusions:
- Functional physical tests can alter plasma nitrite levels in older adults.
- These alterations in nitric oxide (NO) bioavailability may have implications for functional health in this population.
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