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Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
Published on: September 6, 2014
Single-gene transgenic mouse strains for reprogramming adult somatic cells
Bryce W Carey1, Styliani Markoulaki, Caroline Beard
1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.
Nature Methods
|December 17, 2009
Summary
Researchers developed transgenic mice for efficient induced pluripotent stem cell (iPSC) generation. This model uses a single drug-inducible transgene to express reprogramming factors, simplifying iPSC production from various somatic cells.
Area of Science:
- Genetics and Genomics
- Stem Cell Biology
- Transgenic Technology
Background:
- Induced pluripotent stem cells (iPSCs) hold promise for regenerative medicine.
- Generating iPSCs typically requires complex multi-gene delivery systems.
- Efficient and simplified methods for iPSC generation are needed.
Purpose of the Study:
- To create a novel transgenic mouse model for simplified iPSC generation.
- To demonstrate the efficacy of a single drug-inducible transgene for reprogramming.
- To facilitate the transfer of reprogramming technology into diverse genetic backgrounds.
Main Methods:
- Development of transgenic mouse models with a single genomic locus for reprogramming factors.
- Utilizing a drug-inducible (doxycycline) transgene for controlled factor expression.
- Culture of various somatic cell types in the presence of doxycycline to induce reprogramming.
Main Results:
- Successfully generated induced pluripotent stem cells (iPSCs) from multiple somatic cell types.
- Demonstrated efficient reprogramming using a single polycistronic reprogramming construct.
- Established that the transgenic system is easily maintained and transferable.
Conclusions:
- The developed transgenic mouse model offers a simplified and efficient method for generating iPSCs.
- This technology streamlines the process of somatic cell reprogramming.
- The system's adaptability allows for its application across different mouse genetic backgrounds.
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