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Updated: Jun 17, 2026

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
Published on: September 6, 2014
Single-gene transgenic mouse strains for reprogramming adult somatic cells
Bryce W Carey1, Styliani Markoulaki, Caroline Beard
1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.
Abstract:
We report transgenic mouse models in which three or four reprogramming factors are expressed from a single genomic locus using a drug-inducible transgene. Multiple somatic cell types can be directly reprogrammed to generate induced pluripotent stem cells (iPSCs) by culture in doxycycline. Because reprogramming factors are carried on a single polycistronic construct, the mice can be easily maintained, and the transgene can be easily transferred into other genetic backgrounds.
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