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Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Published on: December 16, 2016

A reprogrammable mouse strain from gene-targeted embryonic stem cells.

Matthias Stadtfeld¹, Nimet Maherali, Marti Borkent

¹Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, Harvard Stem Cell Institute, Boston, Massachusetts, USA.

|December 17, 2009

Summary

This summary is machine-generated.

Researchers developed

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Generation of Mice Derived from Induced Pluripotent Stem Cells

Generation of Mice Derived from Induced Pluripotent Stem Cells

Published on: November 29, 2012

Area of Science:

Stem cell biology
Genetics
Molecular biology

Background:

Induced pluripotent stem cells (iPSCs) are typically generated using viral methods.
Viral delivery can pose risks and limitations in reprogramming somatic cells.

Purpose of the Study:

To create a novel genetic tool for studying cellular reprogramming.
To develop a safer and more efficient method for generating iPSCs.

Main Methods:

Gene targeting was employed to create a mouse model.
A single-copy inducible, polycistronic reprogramming cassette was integrated into the mouse genome.
This allows for controlled induction of pluripotency in various somatic cell types.

Main Results:

A new strain of 'reprogrammable mice' was successfully generated.
The system allows for the induction of pluripotency in diverse somatic cells.
These mice are easily bred, facilitating further research.

Conclusions:

The developed 'reprogrammable mice' offer a valuable, non-viral tool for studying reprogramming mechanisms.
This genetic approach simplifies the generation and study of iPSCs.
The model system aids in understanding the fundamental processes of cellular reprogramming.