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Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

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Related Experiment Video

Updated: Jun 17, 2026

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

EOS lentiviral vector selection system for human induced pluripotent stem cells.

Akitsu Hotta¹, Aaron Y L Cheung, Natalie Farra

¹Developmental and Stem Cell Biology Program, SickKids, Toronto, Ontario, Canada.

Nature Protocols

|December 17, 2009

Summary

Generating induced pluripotent stem (iPS) cells is crucial for disease research and therapies. This study details a protocol for a lentiviral selection system to efficiently enrich for human iPS cells, overcoming reprogramming challenges.

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Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

Published on: October 31, 2012

Area of Science:

Stem Cell Biology
Molecular Medicine
Biotechnology

Background:

Induced pluripotent stem (iPS) cells offer significant potential for disease modeling, drug screening, and regenerative medicine.
Low efficiency and heterogeneity in reprogramming hinder the generation of patient-specific iPS cell lines.
Previous work established the first selection system for enriched reprogrammed human iPS cells.

Purpose of the Study:

To provide a detailed protocol for producing a pluripotent state-specific lentiviral vector.
To describe a selection system for inducing healthy and disease-specific human iPS cells.
To improve the efficiency and consistency of human iPS cell generation.

Main Methods:

Development of a lentiviral vector expressing enhanced green fluorescence protein (eGFP) and puromycin resistance.
Utilizing the vector to mark and select for human iPS cell colonies expressing endogenous pluripotency markers.
Detailed protocol preparation for the lentiviral vector and selection system.

Main Results:

Successful development of a lentiviral selection system for enriched human iPS cells.
Demonstration of marking and enriching iPS cell colonies based on pluripotency markers.
Established protocol for efficient generation of iPS cells.

Conclusions:

The described protocol enables efficient production of the selection system in 2 weeks.
Human iPS cell generation using this system takes approximately 2 months.
This method facilitates the generation of personalized iPS cell lines for research and therapeutic applications.