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Excreted/secreted proteins from trypanosome procyclic strains
Celestine Michelle Atyame Nten1, Nicolas Sommerer, Valerie Rofidal
1UMR 177, IRD-CIRAD, CIRAD TA A-17 / G, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France.
Journal of Biomedicine & Biotechnology
|December 17, 2009
Summary
Researchers identified proteins secreted by Trypanosoma parasites, crucial for virulence and transmission. This study reveals key proteins in Trypanosoma brucei gambiense and Trypanosoma brucei brucei, offering targets for disease control.
Area of Science:
- Parasitology
- Molecular Biology
- Biochemistry
Background:
- The secretome of Trypanosoma parasites plays a role in virulence.
- Secreted proteins are suspected to influence parasite life cycle stages, including Glossina midgut establishment and metacyclogenesis.
Purpose of the Study:
- To identify proteins secreted by procyclic strains of T. brucei gambiense and T. brucei brucei.
- To understand the role of these secreted proteins in human and animal trypanosomiasis.
Main Methods:
- Mass spectrometry was employed to characterize the secretomes of T. brucei brucei and T. brucei gambiense.
- Proteins were identified and quantified to determine species-specific and common components.
Main Results:
- 427 and 483 nonredundant proteins were identified in T. brucei brucei and T. brucei gambiense secretomes, respectively.
- 35% and 42% of proteins were specific to T. brucei brucei and T. brucei gambiense, respectively; 279 proteins were common to both.
- Proteins were classified into 12 functional classes, with a focus on 14 families of abundant proteases implicated in infection and nutrition.
- Evidence suggests the involvement of the exosome pathway in trypanosome extracellular space secretion.
Conclusions:
- The study provides a comprehensive characterization of T. brucei secretomes.
- Identified proteins, particularly proteases, offer potential targets for developing novel strategies to block trypanosome transmission.
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