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Updated: Jun 17, 2026

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
Published on: July 4, 2016
Distance and dynamics determination by W-band DEER and W-band ST-EPR
Likai Song1, Mioara Larion, Jean Chamoun
1National High Magnetic Field Laboratory, Institute of Molecular Biophysics, Department of Biological Science, Department of Biochemistry and Chemistry, Florida State University, Tallahassee, FL 32310, USA.
Abstract:
To explore high-field EPR in biological applications we have compared measurements of dynamics with X-band (9 GHz) and W-band (94 GHz) saturation transfer EPR (ST-EPR) and distance determination by X and W-band DEER. A fourfold increase of sensitivity was observed for W-band ST-EPR compared with X-band. The distance measurements at both fields showed very good agreement in both the average distances and in the distance distributions. Multifrequency EPR thus provides an additional experimental dimension to facilitate extraction of distance populations. However, the expected orientational selectivity of W-band DEER to determine the relative orientation of spins has not been realized, most likely because of the large orientational disorder of spin labels on the protein surface.
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