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Seven novel single nucleotide polymorphisms identified within river buffalo (Bubalus bubalis) lactoferrin gene
Periasamy Kathiravan1, Ranjit S Kataria, Bishnu P Mishra
1National Bureau of Animal Genetic Resources, P.B. No. 129, GT Road Bypass, Karnal, Haryana, 132 001, India. kathirvet@yahoo.co.in
Tropical Animal Health and Production
|December 17, 2009
Summary
Researchers identified seven single-nucleotide polymorphic (SNP) sites in the lactoferrin gene of Indian riverine buffaloes. These SNPs may help in breeding buffaloes resistant to mastitis.
Area of Science:
- Genetics
- Animal Science
- Molecular Biology
Background:
- The lactoferrin gene plays a crucial role in immune function.
- Identifying genetic variations in buffaloes can aid in disease resistance breeding programs.
Purpose of the Study:
- To identify single-nucleotide polymorphic (SNP) sites within coding and non-coding regions of the bubaline lactoferrin gene.
- To explore the potential of these SNPs as genetic markers for mastitis susceptibility in Indian riverine buffaloes.
Main Methods:
- Screening of 102 buffaloes from six breeds across six lactoferrin gene loci.
- Utilizing Single-Strand Conformation Polymorphism (SSCP) analysis.
- Performing sequence analysis of identified SSCP variants.
Main Results:
- Eight distinct patterns were observed at three lactoferrin gene loci (LtfE5, LtfE10, LtfE16).
- Two synonymous SNP sites were identified in the coding region (exon 16).
- Five SNP sites were found in flanking non-coding regions (intron 4 and intron 9).
Conclusions:
- The identified SNPs and haplotypes in the lactoferrin gene are potential markers.
- These markers could be associated with mastitis resistance or susceptibility in buffalo populations.
- This research contributes to marker-assisted selection for improved buffalo health.
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