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Collagen-induced arthritis in mice
Lisette Bevaart1, Margriet J Vervoordeldonk, Paul P Tak
1Division of Clinical Immunology and Rheumatology, Academic Medical Center/University of Amsterdam, Amsterdam, The Netherlands.
Methods in Molecular Biology (Clifton, N.J.)
|December 17, 2009
Summary
This study details protocols for inducing collagen-induced arthritis (CIA) in DBA/1 and C57BL/6 mice, crucial models for rheumatoid arthritis (RA) research. It also covers monitoring arthritis progression and detecting collagen-specific antibodies.
Area of Science:
- Immunology
- Rheumatology
- Animal Models
Background:
- Collagen-induced arthritis (CIA) is a key animal model for studying rheumatoid arthritis (RA).
- The CIA model is vital for investigating arthritis development mechanisms and evaluating novel therapeutics.
- Recent advancements allow CIA induction in C57BL/6 mice, enabling research with knockout strains.
Purpose of the Study:
- To provide detailed protocols for inducing CIA in DBA/1 and C57BL/6 mice.
- To describe methods for monitoring clinical signs of arthritis and paw swelling.
- To outline procedures for paw decalcification and detection of collagen-specific antibodies.
Main Methods:
- Induction of CIA in DBA/1 and C57BL/6 mice using specific protocols.
- Clinical monitoring of arthritis severity and paw edema.
- Histopathological analysis including decalcification of paws.
- Serological analysis for collagen-specific antibody detection.
Main Results:
- Established protocols for reliable CIA induction in two distinct mouse strains.
- Demonstrated methods for quantitative assessment of arthritis development.
- Validated techniques for antibody detection relevant to CIA.
Conclusions:
- The described protocols facilitate robust CIA model generation in DBA/1 and C57BL/6 mice.
- These methods support comprehensive investigation of RA pathogenesis and therapeutic interventions.
- The protocols enable the use of genetically modified mice for advanced arthritis research.
