Related Experiment Video
Updated: Jun 17, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
Published on: April 4, 2025
Computational approaches to the detection and analysis of sequences with intramolecular G-quadruplex forming
Paul Ryvkin1, Steve G Hershman, Li-San Wang
1Department of Pathology, Penn Center for Bioinformatics, and Graduate Group in Genomics and Computational Biology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA.
Abstract:
Sequences with the potential to form intramolecular G-quadruplexes (G4-structures) are found in highly nonrandom distributions in the genomes of diverse organisms. These sequences are associated with nucleic acid metabolic processes ranging from transcription and translation to recombination and telomere function. Here we review different computational methods for identifying potential G4-forming sequences and provide protocols for their implementation. We also discuss methods for assessing the significance and specificity of associations between the sequences and different biological functions.
Related Concept Videos
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...
Sanger Sequencing

