Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differential membrane recognition by RNA and DNA G-quadruplexes governed by lipid composition and structural topology.

International journal of biological macromolecules·2026
Same author

Fatty Acid-Induced Modulation of Saliva Protein-Polyphenol Interactions: Molecular Signatures Underlying the Camembert Effect of Astringency.

Biomacromolecules·2026
Same author

Automated Baseline-Correction and Signal-Detection Algorithms with Web-Based Implementation for Thermal Liquid Biopsy Data Analysis.

Cancers·2026
Same author

Digging for cold: how temperature and depth determine hibernacula selection for a small mammalian hibernator, the New Mexico Jumping Mouse (<i>Zapus luteus</i>).

Journal of mammalogy·2025
Same author

Deciphering the intermolecular interactions between G-quadruplex (G4)-forming sequences.

Nucleic acids research·2025
Same author

Adaptive Physics-Aware Raman Baseline Correction with Machine Learning Predicted Parameters.

Analytical chemistry·2025

Related Experiment Video

Updated: Jun 17, 2026

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines
05:32

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

Published on: May 12, 2023

2-aminopurine as a probe for quadruplex loop structures.

Robert D Gray1, Luigi Petraccone, Robert Buscaglia

  • 1James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 17, 2009
PubMed
Summary
This summary is machine-generated.

2-aminopurine, a fluorescent adenine analog, effectively probes quadruplex DNA loop structures. Its emission intensity and quenching sensitivity help compare crystal and solution structures of telomeric DNA models.

More Related Videos

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

Related Experiment Videos

Last Updated: Jun 17, 2026

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines
05:32

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

Published on: May 12, 2023

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biophysics

Background:

  • Fluorescent reporter groups are valuable for studying macromolecular structure and conformational changes.
  • Intrinsic fluorescence of nucleic acids is generally too low for structural studies.
  • 2-aminopurine is a highly fluorescent adenine analog used as a reporter group in polynucleotide studies.

Purpose of the Study:

  • To utilize 2-aminopurine as a probe for investigating loop structures in quadruplex DNA.
  • To demonstrate the utility of 2-aminopurine in comparing crystal and solution structures of oligonucleotide models.
  • To discriminate between structural models of telomeric quadruplexes with tandem repeats.

Main Methods:

  • Incorporation of 2-aminopurine as a fluorescent reporter group into DNA.
  • Spectroscopic analysis of 2-aminopurine fluorescence, including emission intensity.
  • Assessment of 2-aminopurine's sensitivity to exogenous quenching molecules like acrylamide.

Main Results:

  • 2-aminopurine fluorescence provides sensitive detection of structural nuances in quadruplex DNA.
  • Comparative analysis of 2-aminopurine emission aids in evaluating crystal versus solution structures.
  • Quenching studies with acrylamide help differentiate between structural models of telomeric quadruplexes.

Conclusions:

  • 2-aminopurine is a powerful tool for probing quadruplex DNA loop structures.
  • Fluorescence spectroscopy with 2-aminopurine enables detailed comparison of DNA structures in different states.
  • This approach aids in the structural elucidation of complex DNA architectures like telomeric quadruplexes.