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Endothelial Cell Co-culture Mediates Maturation of Human Embryonic Stem Cell to Pancreatic Insulin Producing Cells in a Directed Differentiation Approach
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Decrease in expression of histamine H2 receptors by human amniotic epithelial cells during differentiation into

D Szukiewicz1, M Pyzlak, A Stangret

  • 1Department of General and Experimental Pathology, Medical University of Warsaw, Warsaw, Poland. Dszukiewicz@hotmail.com

Inflammation Research : Official Journal of the European Histamine Research Society ... [Et Al.]
|December 17, 2009
PubMed
Summary
This summary is machine-generated.

Histamine H(2) receptor expression decreases during nicotinamide-induced differentiation of human amniotic epithelial cells into pancreatic-like cells. This change may signal histamine

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In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Published on: November 28, 2019

Area of Science:

  • Stem cell biology
  • Histamine receptor research
  • Cell differentiation

Background:

  • The histaminergic pathway plays a role in stem cell differentiation.
  • Human amniotic epithelial cells (HAEC) can differentiate into pancreatic-like cells (PBLC).
  • Histamine receptors H(1) and H(2) are involved in cellular processes.

Purpose of the Study:

  • To compare H(1) and H(2) receptor expression during nicotinamide-induced differentiation of HAEC into PBLC.
  • To investigate the role of the histaminergic pathway in this differentiation process.

Main Methods:

  • HAEC were cultured and induced to differentiate using nicotinamide.
  • C-peptide levels were measured as a marker of differentiation.
  • Quantitative immunohistochemistry was used to evaluate H(1) and H(2) receptor expression at different time points.

Main Results:

  • Nicotinamide successfully induced differentiation, evidenced by increased C-peptide levels.
  • H(2) receptor expression remained unchanged initially but significantly decreased after Day 9.
  • H(1) receptor expression patterns were not detailed in the abstract.

Conclusions:

  • Variable H(2) receptor expression during differentiation suggests histamine involvement.
  • The observed decrease in H(2) may indicate a specific time-point for histamine's role in early differentiation stages.