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Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Oxidized low-density lipoprotein.
Sampath Parthasarathy1, Achuthan Raghavamenon, Mahdi Omar Garelnabi
1Division of Cardiothoracic Surgery, The Ohio State University Medical Center, Columbus, OH, USA.
Methods in Molecular Biology (Clifton, N.J.)
|December 17, 2009
Summary
Oxidized low-density lipoprotein (Ox-LDL) remains poorly defined despite 25 years of study. This review details Ox-LDL components, biological actions, and preparation methods, highlighting its complex and variable nature.
Area of Science:
- Biochemistry
- Cardiovascular Research
- Lipid Metabolism
Background:
- Oxidized low-density lipoprotein (Ox-LDL) has been investigated for over two decades for its role in atherosclerosis.
- Despite extensive research, Ox-LDL lacks a precise definition and characterization due to its variable composition.
- Its in vivo form and pathophysiological significance are not fully understood.
Purpose of the Study:
- To review the diverse components of Ox-LDL.
- To discuss the known biological actions of these components.
- To outline various methods used for Ox-LDL preparation.
Main Methods:
- Literature review of studies on Ox-LDL composition, biological effects, and preparation techniques.
- Analysis of the variability in Ox-LDL based on source, preparation, storage, and usage.
- Synthesis of information on oxidized fatty acid derivatives, cholesterol oxidation products, and modified proteins within Ox-LDL.
Main Results:
- Ox-LDL is a heterogeneous mixture containing oxidized lipids, cholesterol oxidation products, and modified proteins.
- Component variability is influenced by preparation methods and source materials.
- The exact in vivo structure and formation pathways of Ox-LDL require further elucidation.
Conclusions:
- Ox-LDL is a complex and ill-defined entity with significant implications in cardiovascular disease.
- Understanding its components and preparation is crucial for accurate research.
- Further investigation into its in vivo generation and pathophysiological role is necessary.
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