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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies
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Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

Published on: November 4, 2021

Peptidomics: divide et impera.

Mikhail Soloviev1

  • 1School of Biological Sciences, Royal Holloway University of London, TW20 0EX, Egham, Surrey, UK. mikhail.soloviev@rhul.ac.uk

Methods in Molecular Biology (Clifton, N.J.)
|December 17, 2009
PubMed
Summary
This summary is machine-generated.

Peptidomics systematically analyzes peptides in biological systems. This field involves dividing complex peptide mixtures for structural and functional characterization, offering insights into cellular processes.

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Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies
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Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
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Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

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Area of Science:

  • Biochemistry
  • Proteomics
  • Systems Biology

Background:

  • Peptidomics involves the comprehensive study of peptides within biological entities.
  • It encompasses naturally occurring peptides and peptidomic approaches to protein analysis.
  • The field utilizes a 'divide and conquer' strategy for complex peptide mixtures.

Purpose of the Study:

  • To introduce the fundamental concept of peptidomics.
  • To outline the diverse methodologies used in peptidomic studies.
  • To identify key peptide targets commonly investigated.

Main Methods:

  • Systematic analysis of peptide content in cells, tissues, or organisms.
  • Purification and separation of complex peptidomes.
  • Structural and functional characterization of identified peptides.

Main Results:

  • Peptidomes can contain tens to thousands of functional peptides.
  • The 'divide and conquer' approach is central to peptidomic analysis.
  • Methodologies enable detailed characterization of peptide groups.

Conclusions:

  • Peptidomics provides a systematic framework for peptide analysis.
  • Understanding peptidomes is crucial for deciphering biological functions.
  • The field offers a range of techniques for peptide discovery and characterization.