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Quantifying Synapses: an Immunocytochemistry-based Assay to Quantify Synapse Number
Published on: November 16, 2010
Quantitative neuroproteomics of the synapse.
Dinah Lee Ramos-Ortolaza1, Ittai Bushlin, Noura Abul-Husn
1Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY, USA.
Methods in Molecular Biology (Clifton, N.J.)
|December 17, 2009
Summary
Proteomic analysis of brain tissue offers new insights into neurodegenerative diseases. These methods identify synaptic proteins and quantify their levels under different conditions, aiding in understanding neuronal function.
Area of Science:
- Neuroscience
- Proteomics
- Molecular Biology
Background:
- Neuropsychiatric and neurodegenerative diseases require advanced study methods.
- Proteomic analysis of brain tissue is an emerging technique.
Purpose of the Study:
- To describe methods for isolating and identifying proteins in specific brain samples, like synapses.
- To compare protein levels under various conditions.
Main Methods:
- Subcellular fractionation techniques.
- Quantitative proteomics utilizing isotopic labels.
Main Results:
- Identification of proteins associated with specific cellular compartments.
- Quantification of protein level changes under different experimental conditions.
Conclusions:
- These proteomic methods enhance understanding of neuronal organization.
- The techniques aid in elucidating the regulation of synaptic function in disease states.
