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Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Fluorescence methods for evaluating lipoplex-mediated gene delivery.

Henrique Faneca¹, Nejat Düzgüneş, Maria C Lima

¹Center for Neuroscience and Cell Biology, University of Coimbra, Portugal.

Methods in Molecular Biology (Clifton, N.J.)

|December 17, 2009

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This study explores how cationic liposome/DNA complexes (lipoplexes) function biologically. It details methods using fluorescent probes to analyze DNA protection, cellular interactions, and delivery processes.

Area of Science:

Biotechnology
Molecular Biology
Cell Biology

Background:

Cationic liposome/DNA complexes, or lipoplexes, are crucial for gene delivery.
Their biological activity hinges on DNA protection and cellular interaction mechanisms.

Purpose of the Study:

To outline methods for studying lipoplex biological activity.
To investigate DNA protection, cell surface binding, endocytosis, and cytoplasmic delivery.

Main Methods:

Utilizing fluorescent probes to track lipoplex behavior.
Employing various experimental procedures to analyze cellular uptake and DNA release.

Main Results:

Fluorescent probes enable detailed observation of lipoplex-cell interactions.

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Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

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Published on: July 30, 2020

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Last Updated: Jun 17, 2026

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

Published on: July 30, 2020

The methods allow for quantitative and qualitative assessment of gene delivery efficiency.

Conclusions:

Fluorescent probe-based methods are effective for evaluating lipoplex performance.
Understanding these processes is key to optimizing gene therapy vectors.