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Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
Published on: February 1, 2019
Fluorescence methods for evaluating lipoplex-mediated gene delivery
Henrique Faneca1, Nejat Düzgüneş, Maria C Lima
1Center for Neuroscience and Cell Biology, University of Coimbra, Portugal.
Methods in Molecular Biology (Clifton, N.J.)
|December 17, 2009
Abstract:
The biological activity of cationic liposome/DNA complexes ("lipoplexes") is strongly dependent on their ability to protect DNA and to interact with cells, including binding to the cell surface, internalization via endocytosis and cytoplasmic delivery of the DNA. In this chapter, we describe a number of methods and procedures to study these processes, based on the use of fluorescent probes.
