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Design, synthesis, and amplification of DNA pools for in vitro selection

Bradley Hall1, John M Micheletti, Pooja Satya

  • 1Department of Chemistry and Biochemistry, University of Texas, Austin, Texas, USA.

Current Protocols in Nucleic Acid Chemistry
|December 17, 2009
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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...