Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tight Junctions01:29

Tight Junctions

Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
Adherens Junctions01:24

Adherens Junctions

Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types –  adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as  epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as  heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
The endothelial cells...
Anchoring Junctions01:03

Anchoring Junctions

Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Alpelisib for TEK- and PIK3CA-Related Venous Malformations: A Systematic Review.

The Australasian journal of dermatology·2026
Same author

Increasing the pathology workforce is critical for timely and comprehensive cancer care.

CMAJ : Canadian Medical Association journal = journal de l'Association medicale canadienne·2026
Same author

Camsap2a regulates actomyosin flow and Rab5ab-mediated macropinocytosis in the yolk cell during zebrafish epiboly.

Development (Cambridge, England)·2026
Same author

Triage to Advice Only for Referring Providers: A Pilot Project Using Formal Consensus Methods to Establish Appropriate Patients for Electronic Consultations.

AACE endocrinology and diabetes·2025
Same author

A comprehensive review of data sharing practices in addiction research: Trends, challenges, and future directions.

Drug and alcohol dependence·2025
Same author

A cell fate mapping simulation laboratory to increase undergraduate students' understanding of early developmental processes in frog, zebrafish, and tunicate embryos.

Journal of microbiology & biology education·2025

Related Experiment Video

Updated: Jun 17, 2026

Fluorescence Labeling to Visualize Low-Expressed Proteins in Zebrafish
09:38

Fluorescence Labeling to Visualize Low-Expressed Proteins in Zebrafish

Published on: January 24, 2025

The tight junction component Claudin E is required for zebrafish epiboly.

Manal Siddiqui1, Hasan Sheikh, Christopher Tran

  • 1Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|December 17, 2009
PubMed
Summary

Claudins are crucial for zebrafish epiboly, the process where the embryo spreads to cover the yolk. Claudin E (Cldne) knockdown in the enveloping layer (EVL) disrupts yolk syncytial layer (YSL) attachment, delaying epiboly.

More Related Videos

Microgavage of Zebrafish Larvae
10:53

Microgavage of Zebrafish Larvae

Published on: February 20, 2013

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
10:06

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

Published on: October 19, 2013

Related Experiment Videos

Last Updated: Jun 17, 2026

Fluorescence Labeling to Visualize Low-Expressed Proteins in Zebrafish
09:38

Fluorescence Labeling to Visualize Low-Expressed Proteins in Zebrafish

Published on: January 24, 2025

Microgavage of Zebrafish Larvae
10:53

Microgavage of Zebrafish Larvae

Published on: February 20, 2013

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
10:06

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

Published on: October 19, 2013

Area of Science:

  • Developmental biology
  • Cell biology
  • Molecular biology

Background:

  • Zebrafish epiboly involves blastoderm spreading over the yolk, guided by the yolk syncytial layer (YSL) via tight junctions with the enveloping layer (EVL).
  • Claudins are key transmembrane proteins forming tight junctions, essential for cell adhesion and tissue integrity.

Purpose of the Study:

  • To investigate the specific role of Claudin E (Cldne) in zebrafish epiboly.
  • To elucidate the mechanism by which Cldne influences EVL-YSL interactions during embryonic development.

Main Methods:

  • Morpholino-induced knockdown of Claudin E (cldne) in zebrafish embryos.
  • Analysis of epiboly progression and EVL-YSL attachment dynamics in morphant embryos.

Main Results:

  • Knockdown of cldne significantly delayed zebrafish epiboly.
  • Reduced tension was observed at the EVL margin attached to the YSL in cldne morphants.
  • Uneven EVL and blastoderm advancement suggests impaired EVL-YSL attachment strength.

Conclusions:

  • Claudin E is essential for maintaining proper EVL-YSL attachment strength during zebrafish epiboly.
  • This study provides the first evidence for the critical role of Claudins in regulating zebrafish epiboly progression.