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Related Experiment Video

Updated: Jun 17, 2026

Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography
11:13

Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography

Published on: January 7, 2015

A high-throughput model for fat graft assessment.

Miguel A Medina1, John T Nguyen, Michael M McCormack

  • 1Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital, Boston, Massachusetts 02114, USA. mamedina@partners.org

Lasers in Surgery and Medicine
|December 17, 2009
PubMed
Summary

This study developed a 10-day model to predict fat graft survival by measuring adipocyte apoptosis. Agents like P188 and lipoic acid reduced early apoptosis, correlating with better long-term graft survival and reduced reabsorption.

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Area of Science:

  • Biomedical Engineering
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Current animal models for fat grafting assessment are time-consuming, requiring weeks to months for results.
  • Rapid evaluation of fat graft survival is crucial for developing effective adipocyte protectants and grafting techniques.

Purpose of the Study:

  • To develop a rapid model for identifying adipocyte protectants using apoptosis-specific fluorescence.
  • To predict long-term fat graft survival within a 10-day period.

Main Methods:

  • Human fat was treated with agents including P188, polyethylene glycol (PEG 8000), and lipoic acid (LA), or saline control.
  • Apoptosis levels were measured in fat explants over 10 days and at six weeks.
  • Histology, DNA content, and weight changes were analyzed to assess graft survival and quality.

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Last Updated: Jun 17, 2026

Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography
11:13

Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography

Published on: January 7, 2015

Innovative Adipose Tissue Fractionation for Transforming Fat into Specialized Components
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Innovative Adipose Tissue Fractionation for Transforming Fat into Specialized Components

Published on: July 11, 2025

Engineered Vascularized Muscle Flap
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Main Results:

  • P188 and LA treatments significantly reduced early adipocyte apoptosis compared to controls.
  • Reduced early apoptosis correlated with decreased graft reabsorption (by weight) and increased DNA content at six weeks.
  • PEG 8000 treatment increased apoptosis and graft reabsorption, with histological evidence of inflammation.

Conclusions:

  • A 10-day apoptosis-based model can effectively predict long-term fat graft survival.
  • This model allows for rapid screening of agents and methods to enhance adipocyte survival and graft take.
  • Early apoptosis measurements provide reliable indicators of graft outcomes at six weeks.