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Updated: Jun 17, 2026

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
Published on: September 29, 2011
Free solution hydrodynamic separation of DNA fragments from 75 to 106,000 base pairs in a single run
Xiayan Wang1, Vijaykumar Veerappan, Chang Cheng
1Department of Chemistry and Biochemistry, The University of Oklahoma, Norman, Oklahoma 73019, USA.
Abstract:
Gel electrophoresis is commonly used to separate DNA, but narrow capillaries or microchannels desired for high throughput efficient separations are difficult to fill with gels. We report here that a narrow capillary can be used to hydrodynamically separate a wide size range of DNA fragments in a single run without the need for gels, wall coatings, or an electric field. We also demonstrate that attractive separation is possible in a few minutes and that the separated DNA can be collected into individual fractions that remain viable for amplification via the polymerase chain reaction.
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