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Visualization of Neutrophil Extracellular Traps in Mesenteric Venules After Mesenteric Ischemia-Reperfusion Injury via Intravital Microscopy
Published on: September 27, 2024
Proteomic analysis of post-hemorrhagic shock mesenteric lymph
Jen-Feng Fang1, Lih-Yuann Shih, Kuo-Ching Yuan
1Department of Orthopedics, Chang-Gung Memorial Hospital, Chang-Gung University, Taoyuan, Taiwan. jimfang@adm.cgmh.org.tw
Shock (Augusta, Ga.)
|December 18, 2009
Summary
Mesenteric lymph contains proteins that change after hemorrhagic shock, potentially causing organ damage. Identifying these toxic mediators is key for developing new treatments for shock-induced injuries.
Area of Science:
- Biochemistry
- Physiology
- Proteomics
Background:
- Mesenteric lymph's role in hemorrhagic shock-induced organ failure is documented.
- Mediators and mechanisms of mesenteric lymph toxicity remain largely unknown.
Purpose of the Study:
- Identify potential mediators or biomarkers in mesenteric lymph post-hemorrhagic shock.
- Utilize comparative proteomic analysis to understand lymph composition changes.
Main Methods:
- Rats subjected to trauma and hemorrhagic shock or sham shock.
- Mesenteric lymph collected pre-shock and post-resuscitation.
- Proteomic analysis using 2D gel electrophoresis and MALDI-TOF MS.
Main Results:
- Over 31 protein spots showed >2-fold change in abundance.
- 12 distinct proteins identified; 4 upregulated (albumin, actin, complement C3, major urinary protein), 2 downregulated (haptoglobin, unidentified).
- Protein alterations confirmed by Western blots; many linked to inflammation.
Conclusions:
- Identified specific proteins in mesenteric lymph that are altered after hemorrhagic shock.
- These proteins may play a role in hemorrhagic shock-induced lung injury and organ failure.
- Findings offer potential therapeutic targets for treating shock-related complications.
