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Updated: Jun 17, 2026
Bone Marrow Transplantation Platform to Investigate the Role of Dendritic Cells in Graft-versus-Host Disease
Published on: March 17, 2020
Engineered regulatory T cells prevent graft-versus-host disease while sparing the graft-versus-leukemia effect after
Jiang Cao1, Chong Chen, Lingyu Zeng
1Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, No 99 West Huaihai Road, Xuzhou, Jiangsu Prov 221002, China.
Abstract:
Regulatory T cells (Tregs) can prevent graft-versus-host disease (GVHD) after allogeneic bone marrow transplantation (BMT). Here we developed a lentivirus-based strategy to ectopically express Foxp3 in mouse CD4(+)CD25(-) T cells. These cells shared similar immunophenotypes and biological features of natural Tregs. Co-injection of engineered Tregs with donor bone marrow cells and splenocytes prevented recipients from lethal GVHD. Furthermore, we showed that graft-versus-leukemia (GVL) effect against EL4/DsRed leukemic cells was maximally preserved while GVHD was minimized during exposure to engineered Tregs in a mouse leukemia model. These findings provide a novel approach to preventing GVHD while maintaining GVL effect during BMT.
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