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Related Concept Videos

Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Determination01:51

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...

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A Practical Approach to Genetic Inducible Fate Mapping: A Visual Guide to Mark and Track Cells In Vivo
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Wnt2 regulates progenitor proliferation in the developing ventral midbrain.

Kyle M Sousa1, J Carlos Villaescusa, Lukas Cajanek

  • 1Laboratory of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Scheeles väg 1, A1:2, 17177 Stockholm, Sweden.

The Journal of Biological Chemistry
|December 19, 2009
PubMed
Summary
This summary is machine-generated.

Wnt2 protein is crucial for brain development, specifically regulating dopaminergic neuron growth. This study reveals Wnt2

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Last Updated: Jun 17, 2026

A Practical Approach to Genetic Inducible Fate Mapping: A Visual Guide to Mark and Track Cells In Vivo
13:36

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Published on: December 30, 2009

Modeling Paracrine Noncanonical Wnt Signaling In Vitro
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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Molecular Biology

Background:

  • Wnt proteins are secreted, lipidated signaling molecules essential for numerous developmental processes.
  • Wnt signaling pathways play a critical role in central nervous system development, including the formation of dopaminergic neurons.

Purpose of the Study:

  • To purify Wnt2 protein and analyze its signaling capabilities.
  • To elucidate the function of Wnt2 in the development of dopaminergic neurons in vitro and in vivo.

Main Methods:

  • Purification and biochemical analysis of Wnt2.
  • In vitro studies using ventral midbrain precursor cultures and dopaminergic cell lines (SN4741).
  • In vivo analysis of Wnt2-null mice during development.

Main Results:

  • Purified Wnt2 activates the canonical Wnt/β-catenin pathway by inducing Lrp5/6 and Dvl-2/3 phosphorylation and β-catenin activation in dopaminergic cells.
  • Wnt2 promotes progenitor proliferation and increases the number of dopaminergic neurons in developing ventral midbrain cultures.
  • Wnt2-null mice exhibit reduced progenitor proliferation, neurogenesis, and a consequent decrease in dopaminergic neuron populations.

Conclusions:

  • Wnt2 is identified as a novel and significant regulator of dopaminergic progenitor cells.
  • Wnt2 plays a critical role in the developmental processes leading to the formation of dopaminergic neurons in the ventral midbrain.