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Updated: Jun 17, 2026
Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells
Published on: July 22, 2021
Protective effect of clusterin on oxidative stress-induced cell death of human corneal endothelial cells
Young Joo Shin1, Jeong Hun Kim, Jong Mo Seo
1Myung Dong St. Mary's Eye Center, Seoul National University College of Engineering, Seoul, Korea.
Purpose:
To investigate the protective effect of clusterin on oxidative stress-induced cell death of human corneal endothelial cells.
Methods:
Human corneal endothelial cells (HCECs) were cultured according to previously published methods. With treatment of various concentrations (0-50 mM) of tert-butyl hydroperoxide (tBHP) or clusterin, reactive oxygen species (ROS) producrion was measured using an oxidationsensitive fluorescent probe and 2'7'-dichlorofluorescin diacetate (DCFH-DA). Cell viability was assayed with a Cell Counting Kit-8.
Results:
In HCECs, DCF-DA staining revealed that cells treated with a higher concentration of tBHP had higher fluorescent intensity than cells treated with clusterin, compared to control cells. Clusterin significantly inhibited tBHP-induced ROS production. Cell viability decreased with higher tBHP concentration. Cells treated with clusterin had higher viability than control cells at 5 mM tBHP. Clusterin effectively protected HCECs from ROS-induced cell death.
Conclusions:
Our data suggest that clusterin may protect HCECs from oxidative injury-mediated cell death via inhibition of ROS production.
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