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Related Concept Videos

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

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Related Experiment Video

Updated: Jun 17, 2026

Methylated DNA Immunoprecipitation
21:24

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

Methylation analysis by DNA immunoprecipitation.

Kelsie L Thu1, Larissa A Pikor, Jennifer Y Kennett

  • 1Department of Cancer Genetics and Developmental Biology, British Columbia Cancer Research Centre, 675 West 10th Avenue, Vancouver, BC V5Z 1L3, Canada. kthu@bccrc.ca

Journal of Cellular Physiology
|December 19, 2009
PubMed
Summary
This summary is machine-generated.

DNA methylation patterns are crucial for gene expression and are altered in diseases like cancer. This review focuses on immunoprecipitation (IP)-based methods for analyzing DNA methylation genome-wide.

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Last Updated: Jun 17, 2026

Methylated DNA Immunoprecipitation
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Published on: June 16, 2017

Area of Science:

  • Epigenetics and Molecular Biology
  • Genomics and Bioinformatics

Background:

  • DNA methylation is a key epigenetic mechanism regulating gene expression via chromatin structure.
  • Aberrant DNA methylation patterns are implicated in various diseases, notably human cancers.
  • Genome-wide methylation studies are vital for understanding these alterations.

Purpose of the Study:

  • To review techniques for identifying and quantifying methylated DNA.
  • To emphasize immunoprecipitation (IP)-based platforms for methylation analysis.
  • To discuss critical aspects of genome-wide methylation profiling.

Main Methods:

  • Focus on immunoprecipitation (IP)-based techniques for DNA methylation analysis.
  • Discussion of experimental and bioinformatic approaches for genome-wide data.
  • Consideration of sample handling and data integration strategies.

Main Results:

  • IP-based methods are instrumental for genome-wide DNA methylation studies.
  • Comprehensive analysis requires attention to sample considerations and bioinformatic tools.
  • Integrating DNA methylation data with other genomic data provides deeper insights.

Conclusions:

  • Effective analysis of DNA methylation requires robust methodologies and careful experimental design.
  • IP-based platforms offer powerful tools for genome-wide methylation profiling.
  • Integrating diverse genomic data enhances the understanding of methylation's role in health and disease.