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Updated: Jun 17, 2026

An Assay to Detect Protection of the Retinal Vasculature from Diabetes-Related Death in Mice
Published on: January 12, 2024
Verification of biomarkers for diabetic retinopathy by multiple reaction monitoring
Kyunggon Kim1, Sang Jin Kim, Hyeong Gon Yu
1Department of Biomedical Sciences, Cancer Research Institute, and Genome Research Center for Diabetes and Endocrine Disease, Seoul National University College of Medicine, 28 Yongon-Dong, Seoul 110-799, Korea.
Abstract:
Multiple reaction monitoring was used to verify target proteins in 3 groups of vitreous and plasma samples from 3 stages of diabetic retinopathy: macular hole (nondiabetic control), nonproliferative diabetic retinopathy, and proliferative diabetic retinopathy. Twelve target proteins were quantified using triple quadrupole LC-MS/MS and 3 methods to determine the transitions (information-dependent analysis, the MIDAS workflow, and the PeptideAtlas database). This study might be the first MRM experiment to analyze large numbers of clinical vitreous and plasma samples for biomarker verification. Consequently, several biomarker candidates were identified for use in further applications.

