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Human exposure to selamectin from dogs treated with revolution: methodological consideration for selamectin isolation
R C Gupta1, M B Masthay, T D Canerdy
1Department of Toxicology, Murray State University, Breathitt Veterinary Center, Hopkinsville, KY.
Toxicology Mechanisms and Methods
|December 22, 2009
Summary
Selamectin residue was detected in dog blood 72 hours after topical application. Significant transferable selamectin residue was found on gloves for up to four weeks, posing potential health risks with repeated exposure.
Area of Science:
- Veterinary Pharmacology
- Toxicology
- Analytical Chemistry
Background:
- Revolution, containing selamectin, is a common topical treatment for canine parasites.
- Understanding selamectin residue and transfer is crucial for assessing potential exposure risks.
Purpose of the Study:
- To quantify selamectin residue in canine blood and on handler gloves after topical application.
- To evaluate the duration of transferable selamectin residue on gloves.
Main Methods:
- Topical application of Revolution (240 mg selamectin/dog) to six dogs.
- Collection of blood and glove samples at various time points post-application (0 h to 5 weeks).
- Analysis of selamectin residue using reversed-phase high-performance liquid chromatography (RP-HPLC) with UV detection.
Main Results:
- Selamectin was detected in dog blood only at 72 hours post-application (10.26 +/- 1.06 ng/mL).
- Highest selamectin concentration on gloves was observed at 24 hours (518.90 +/- 66.80 ppm).
- Transferable residue on gloves decreased over time but was detectable for up to four weeks.
Conclusions:
- Selamectin exhibits poor dermal absorption and rapid elimination in dogs.
- Significant amounts of selamectin transfer to gloves, persisting for several weeks.
- Repeated exposure to selamectin residue on gloves may pose health risks to individuals handling treated dogs.
