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Updated: Jun 17, 2026

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates
Published on: February 23, 2018
Automated in vitro dermal absorption (AIDA): development of a cost-effective diffusion cell
Richard P Moody1, Mohammed Akram
1Health Canada, Environmental Health Centre, Systemic Toxicology and Pharmacokinetics Section, Ottawa, Ontario, Canada. rick_moody@hc-sc.gc.ca
Abstract:
A new diffusion cell design is reported for conducting in vitro dermal absorption tests. This disposable cell was inexpensive to construct and reduces the risk of inter-test cross-contamination. A disadvantage was that the polymer material used adsorbed the lipophilic test compound, 14C-nonyl phenol. Data is presented for absorption of tritiated water and 14C-nonyl phenol in a dialysis membrane model, Snakeskin and for tritiated water in human skin (Kp = 6.3 +/- 0.78 cm/h x 10(-3)). Further testing is needed to evaluate the capacity of the 'Dispo' cell for use with lipophilic test chemicals.
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