Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
G-protein Coupled Receptors01:21

G-protein Coupled Receptors

G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
G-protein Coupled Receptors01:21

G-protein Coupled Receptors

G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
G Protein-coupled Receptors01:15

G Protein-coupled Receptors

G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
G Protein-coupled Receptors01:15

G Protein-coupled Receptors

G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
Transducer Mechanism: G Protein–Coupled Receptors01:30

Transducer Mechanism: G Protein–Coupled Receptors

G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
GPCRs are also called heptahelical, 7TM, or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Serotonin 5-HT7 receptor signaling modulates inflammatory responses and survival after myocardial infarction.

Journal of translational medicine·2026
Same author

LPS-induced inflammation differentially affects endogenous Ca<sup>2</sup>⁺ activity in mouse and human iPSC-derived astrocytes.

Molecular medicine (Cambridge, Mass.)·2026
Same author

Neuromuscular dysfunction in patient-derived FUS<sup>R244RR</sup>-ALS iPSC model via axonal downregulation of neuromuscular junction proteins.

NAR molecular medicine·2025
Same author

Combining spatial transcriptomics and ECM imaging in 3D for mapping cellular interactions in the tumor microenvironment.

Cell systems·2025
Same author

miR-7 controls glutamatergic transmission and neuronal connectivity in a Cdr1as-dependent manner.

EMBO reports·2024
Same author

MarShie: a clearing protocol for 3D analysis of single cells throughout the bone marrow at subcellular resolution.

Nature communications·2024

Related Experiment Video

Updated: Jun 17, 2026

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
10:13

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding

Published on: June 9, 2017

G protein--mediated signaling: same receptor, multiple effectors.

Andrew Woehler1, Evgeni G Ponimaskin

  • 1Department of Neuro and Sensory Physiology, University of Göttingen, Göttingen, Germany.

Current Molecular Pharmacology
|December 22, 2009
PubMed
Summary
This summary is machine-generated.

G protein-coupled receptors (GPCRs) initiate complex cell signaling pathways. Research reveals GPCRs can activate multiple G proteins and effectors, and signal independently of G proteins, highlighting intricate cellular communication.

More Related Videos

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators
07:41

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators

Published on: February 20, 2018

Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum
09:40

Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum

Published on: September 20, 2011

Related Experiment Videos

Last Updated: Jun 17, 2026

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
10:13

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding

Published on: June 9, 2017

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators
07:41

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators

Published on: February 20, 2018

Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum
09:40

Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum

Published on: September 20, 2011

Area of Science:

  • Molecular Biology
  • Cellular Signaling
  • Pharmacology

Background:

  • G protein-coupled receptors (GPCRs) are the largest family of cell surface receptors.
  • GPCRs are crucial targets for a significant number of pharmaceuticals.
  • Understanding GPCR structure and function has evolved significantly, revealing complex signal transduction.

Purpose of the Study:

  • To review the foundational knowledge of GPCR signaling.
  • To explore the recent discoveries uncovering the complexity of GPCR signal transduction.
  • To discuss the implications of GPCR oligomerization and specific receptor signaling pathways.

Main Methods:

  • Literature review of established and emerging GPCR research.
  • Analysis of fundamental principles in GPCR signal transduction.
  • Case study of serotonin receptors (5-HT4 and 5-HT7) signaling cascades.

Main Results:

  • GPCR signaling is not a simple linear pathway but involves complex networks.
  • One GPCR can couple to multiple G proteins, activating diverse pathways.
  • G proteins can activate multiple effectors, and GPCRs can signal via G protein-independent routes.
  • GPCR oligomerization influences signaling complexity.

Conclusions:

  • The emerging paradigm of GPCR signaling emphasizes multi-pathway activation and G protein-independent signaling.
  • Serotonin receptors 5-HT4 and 5-HT7 exemplify the intricate nature of GPCR signaling.
  • Further research into GPCR complexity is vital for drug development and understanding cellular processes.