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Cell Specific Gene Expression

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

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Related Experiment Video

Updated: Jun 17, 2026

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)

Published on: June 10, 2020

Reduced expression of cenp-e in human hepatocellular carcinoma.

Zijie Liu¹, Kang Ling, Xia Wu

¹The key laboratory of laboratory medical diagnostics, ministry of education; the faculty of laboratory medicine, Chongqing Medical University, Chongqing PR China. redjacky502@yahoo.com.cn

Journal of Experimental & Clinical Cancer Research : CR

|December 22, 2009

Summary

Reduced CENP-E expression in hepatocellular carcinoma (HCC) correlates with aneuploidy. Low CENP-E levels in liver cells lead to chromosome separation errors, highlighting its role in cancer.

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Area of Science:

Cell Biology
Molecular Oncology
Genetics

Background:

Centromere protein E (CENP-E) is vital for spindle checkpoint function.
Disrupted CENP-E expression can cause chromosome mis-segregation and aneuploidy, a common feature in cancers like hepatocellular carcinoma (HCC).

Purpose of the Study:

To investigate CENP-E expression levels in human HCC.
To determine the impact of reduced CENP-E on chromosome separation in normal liver cells (LO2).

Main Methods:

Quantitative PCR and Western blot were used to measure CENP-E mRNA and protein in HCC tissues, HepG2 cells, and LO2 cells.
CENP-E was knocked down in LO2 cells using shRNA to assess its effect on chromosome segregation via aneuploidy counts.

Main Results:

CENP-E mRNA and protein levels were significantly lower in HCC tissues and HepG2 cells compared to adjacent normal tissues and LO2 cells.
Knockdown of CENP-E in LO2 cells led to a significant increase in aneuploid cells compared to controls.

Conclusions:

CENP-E expression is downregulated in human HCC.
Reduced CENP-E expression directly contributes to aneuploidy in liver cells, suggesting its role in HCC development.