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Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
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Rous Sarcoma Virus (RSV) and Cancer01:03

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RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...

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[Expression and function of VAP-33 in murine dendritic cell sarcoma].

Zhen-li Yang1, Yu-qin Liu, Xiao-cui Bian

  • 1Department of Pathology, Institute of Basic Medical Science, Chinese Academy of Medical Science & School of Basic Medicine, Peking Union Medical College, Beijing 100005, China.

Zhonghua Bing Li Xue Za Zhi = Chinese Journal of Pathology
|December 22, 2009
PubMed
Summary

Vesicle-associated membrane protein 33 (VAP-33) is expressed in dendritic cell sarcoma (DCS) and involved in phagocytosis and glucose transport. Its expression decreases with antigen stimulation but increases with insulin, alongside glucose transporter 4 (GLUT-4).

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Area of Science:

  • Cell Biology
  • Immunology
  • Cancer Research

Background:

  • Dendritic cell sarcoma (DCS) is a rare malignancy. The role of VAP-33 in DCS remains largely unexplored.
  • Understanding VAP-33's function in DCS may reveal novel therapeutic targets.

Purpose of the Study:

  • To investigate the expression and function of VAP-33 in a dendritic cell sarcoma (DCS) cell line.
  • To explore VAP-33's role in cellular processes like phagocytosis and glucose transport.

Main Methods:

  • Mass spectrometry with immunoprecipitation identified VAP-33 in DCS cells.
  • Antigen and insulin stimulation assays were performed.
  • Cell morphology, phagocytosis, and protein expression (VAP-33, GLUT-4) were analyzed using immunofluorescence, confocal microscopy, and Western blotting.

Main Results:

  • VAP-33 was detected in the cytoplasm and cell membrane of DCS cells.
  • Antigen stimulation enhanced DCS cell phagocytosis and altered morphology, while decreasing VAP-33 expression.
  • Insulin stimulation increased VAP-33 and glucose transporter 4 (GLUT-4) expression, with both proteins co-localizing.

Conclusions:

  • VAP-33 expression in DCS, originating from dendritic cells, is linked to vesicle transport during antigen processing.
  • VAP-33 and GLUT-4 play a role in glucose transport within DCS cells.