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Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

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Understanding and mitigating conductivity transitions in weak cation exchange chromatography.

Jace Fogle¹, Jenny Hsiung

¹Genentech Process Research and Development, 1 DNA Way MS 75A, South San Francisco, CA 94080, USA. fogle.jace@gene.com

Journal of Chromatography. A

|December 22, 2009

Summary

Large conductivity fluctuations during high pH washes in weak cation exchange chromatography can impact protein retention. Optimizing buffer concentration and wash duration mitigates these transitions without affecting process performance.

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Area of Science:

Biochemistry
Chemical Engineering
Chromatography

Background:

High pH washes in weak cation exchange chromatography can cause significant conductivity fluctuations.
These fluctuations can negatively impact target protein retention and overall process performance.

Purpose of the Study:

To investigate the causes of large conductivity fluctuations during high pH washes in weak cation exchange chromatography.
To identify factors influencing conductivity transitions and develop mitigation strategies.

Main Methods:

Monitoring conductivity and pH during high pH washes.
Investigating the effects of wash buffer concentration, resin ligand density, and resin ligand pK.
Utilizing a sodium-ion selective electrode to measure effluent counterion concentrations.
Comparing experimental counterion data with ion exchange equilibrium theory.

Main Results:

Observed conductivity drops during pH increase and rises during pH decrease, exceeding 6mS/cm in some cases.
Demonstrated that wash buffer concentration, resin ligand density, and pK significantly affect conductivity transition magnitude.
Validated ion exchange equilibrium theory in predicting counterion exchange during washes.

Conclusions:

Conductivity transitions during high pH washes are influenced by buffer concentration, resin properties, and pH changes.
Optimizing the balance between wash buffer concentration and duration effectively mitigates conductivity fluctuations.
Mitigation strategies can be implemented without compromising the performance of weak cation exchange chromatography processes.