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LDL and HDL subclasses in acute ischemic stroke: prediction of risk and short-term mortality
Aleksandra Zeljkovic1, Jelena Vekic, Vesna Spasojevic-Kalimanovska
1Institute of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, and Special Hospital for Prevention and Therapy of Cerebrovascular Disease Sveti Sava, Belgrade, Serbia. aleksandra.zeljkovic@pharmacy.bg.ac.rs
Insights
Small, dense low-density lipoprotein (sdLDL) particles are linked to increased risk of acute ischemic stroke (AIS). Higher sdLDL levels also predict short-term mortality after AIS, independent of other risk factors.
Area of Science:
- Cardiovascular Research
- Lipid Metabolism
- Neurology
Background:
- Small, dense low-density lipoprotein (sdLDL) and small-sized high-density lipoprotein (HDL) particles are known risk factors for ischemic heart disease.
- The clinical significance of these lipoprotein subclasses for acute ischemic stroke (AIS) remains uncertain.
Purpose of the Study:
- To evaluate the association of LDL and HDL particle sizes and subclasses with AIS risk.
- To assess the relationship between lipoprotein particle characteristics and short-term mortality following AIS.
Main Methods:
- Gradient gel electrophoresis and standard laboratory methods were used to analyze LDL and HDL particles in 200 AIS patients and 162 controls.
- Baseline characteristics of LDL and HDL particles were assessed for their predictive value for AIS and mortality.
Main Results:
- AIS patients exhibited increased levels of sdLDL (LDL III and IVb) and smaller HDL particles (more HDL 3a, 3b, 3c; less HDL 2b).
- Elevated sdLDL was a significant predictor of AIS (OR=4.31) and in-hospital mortality (OR=5.50), even after adjusting for conventional risk factors.
- sdLDL levels were significantly higher in AIS fatalities compared to survivors.
Conclusions:
- Acute ischemic stroke (AIS) is associated with unfavorable distributions of LDL and HDL subclasses.
- Increased sdLDL particles are independently associated with both AIS onset and subsequent short-term mortality.
Objective:
Small, dense low-density lipoprotein (sdLDL) and small-sized high-density lipoprotein (HDL) particles are established risk factors for ischemic heart disease. However, their clinical significance for acute ischemic stroke (AIS) is uncertain. This study evaluates associations of LDL and HDL particle sizes and subclasses with AIS risk and short-term mortality after AIS.
Methods:
Two hundred AIS patients hospitalised for first-in-a-lifetime stroke and 162 apparently healthy controls were included in the study. LDL and HDL particles were separated by gradient gel electrophoresis and serum lipid parameters were measured by standard laboratory methods. Baseline characteristics of LDL and HDL particles were evaluated for the prediction of AIS and short-term mortality after AIS.
Results:
AIS patients had significantly more LDL III and IVb, but less LDL I and II particles. They also had significantly smaller HDL size, more HDL 3a, 3b and 3c and less HDL 2b subclasses. The relative content of both sdLDL and small-sized HDL particles was significantly increased in patients (P<0.001 and P<0.001, respectively). In addition, sdLDL was significantly higher in AIS fatalities (n=25) compared with survivors (n=175, P<0.05). Increased sdLDL was a significant predictor of AIS (OR=4.31; P<0.001) and in-hospital mortality after AIS (OR=5.50; P<0.05). The observed relationships persisted after adjustment for conventional risk factors.
Conclusions:
AIS is associated with adverse distributions of LDL and HDL subclasses. In addition, short-term mortality after AIS is associated with increased sdLDL particles. Our results indicate that sdLDL is an independent predictor of both AIS onset and consecutive short-term mortality.
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