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Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
13:08

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Published on: September 9, 2012

Circulating microparticles are elevated in carriers of factor V Leiden

Anoop K Enjeti1, Lisa F Lincz, Fiona E Scorgie

  • 1Hunter Haematology Research Group, Calvary Mater Newcastle, Edith Street, Waratah, NSW 2298, Australia. Anoop.Enjeti@mater.health.nsw.gov.au

Thrombosis Research
|December 22, 2009
PubMed
Abstract

Insights

Elevated microparticles (MPs), particularly from leukocytes, are found in Factor V Leiden mutation carriers. These increased MPs may contribute to thrombosis risk in heterozygotes.

Area of Science:

  • Hematology
  • Thrombosis Research
  • Molecular Genetics

Background:

  • Microparticles (MPs) are cell-derived fragments implicated in thrombosis.
  • Factor V Leiden (FVL) mutation heterozygotes exhibit variable thrombosis risk.
  • The role of circulating MPs in FVL heterozygote thrombosis remains unclear.

Purpose of the Study:

  • To investigate if elevated circulating microparticle numbers or procoagulant potential contribute to thrombosis risk in Factor V Leiden heterozygotes.

Main Methods:

  • Enumerated circulating platelet, endothelial, and leukocyte MPs via flow cytometry in 45 FVL heterozygotes and controls.
  • Assessed procoagulant potential using enzyme-linked immunoassay for prothrombinase activity and dilute Russell Viper venom test.

Main Results:

  • FVL heterozygotes showed significantly higher levels of circulating MPs compared to controls (p=0.0021).
  • All MP subsets, especially leukocyte-derived MPs (CD45+), were elevated in the FVL group.
  • No significant difference in prothrombinase activity or clotting time was observed between groups; MP levels did not correlate with thrombosis history within the FVL cohort.

Conclusions:

  • Circulating platelet and leukocyte microparticles are elevated in Factor V Leiden heterozygotes.
  • These elevated MPs may be significant contributors to thrombosis risk in carriers of the FVL mutation.

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