Related Experiment Video
Updated: Jun 17, 2026
09:51
Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
Published on: May 18, 2018
Canine CXCL7 and its functional expression in dendritic cells undergoing maturation
Yu-Shan Wang1, Kuang-Wen Liao2, Mo-Fen Chen3
1Animal Cancer Center, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan, ROC; Department of Radiation Therapy and Oncology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan, ROC.
Veterinary Immunology and Immunopathology
|December 22, 2009
Summary
Canine dendritic cells (DCs) produce CXCL7, a neutrophil attractant, when stimulated by inflammatory cytokines. This finding highlights the role of DCs in immune responses by recruiting neutrophils to sites of inflammation.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- CXCL7 (platelet basic protein) is a CXC chemokine that attracts and activates neutrophils.
- Dendritic cells (DCs) are crucial for antigen presentation, but their role in neutrophil recruitment via chemokine production is poorly understood.
- Understanding DC chemokine production is vital for deciphering inflammatory and immune responses.
Purpose of the Study:
- To clone and characterize canine CXCL7.
- To investigate the expression and regulation of CXCL7 in canine dendritic cells (DCs).
- To determine the functional activity of canine CXCL7 in neutrophil migration.
Main Methods:
- Cloning of canine CXCL7 cDNA using 3'RACE from LPS-treated peripheral blood mononuclear cells.
- Neutrophil migration assays using CXCL7-transfected cell supernatants.
- Quantitative analysis of CXCL7 mRNA and protein expression in DCs stimulated with various cytokines (LPS, IL-1beta, IL-6, TGF-beta, TNF-alpha, IFN-gamma) via real-time RT-PCR and Western blotting.
Main Results:
- Canine CXCL7 cDNA was successfully cloned, encoding a protein with chemoattractive activity for neutrophils.
- Canine monocyte-derived DCs expressed CXCL7 upon stimulation.
- Pro-inflammatory cytokines (IL-1beta, IL-6, TNF-alpha, TGF-beta) significantly upregulated CXCL7 mRNA and protein levels in DCs compared to IFN-gamma or LPS.
Conclusions:
- Dog DCs express high levels of the neutrophil chemotactic factor CXCL7 when stimulated by pro-inflammatory cytokines.
- Canine DCs may play a significant role in modulating inflammatory responses by recruiting neutrophils.
- This study provides novel insights into DC-mediated neutrophil recruitment in dogs.
Related Concept Videos
01:22T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
01:24T Cell Types and Functions
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
01:24B Cell Activation and Differentiation
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...