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Simultaneous determination of five-type hepatitis virus antigens in 5 min using an integrated automatic
Dianping Tang1, Juan Tang, Biling Su
1Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education), and Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, China. dianping.tang@hotmail.com
A novel electrochemical immunosensor array enables simultaneous detection of five hepatitis virus antigens in just 5 minutes. This rapid, cost-effective diagnostic tool offers high sensitivity and precision for point-of-care testing.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Immunotechnology
Background:
- Hepatitis viruses pose significant global health challenges, necessitating rapid and accurate diagnostic methods.
- Current diagnostic approaches for multiple hepatitis types can be time-consuming and complex.
- There is a need for integrated, high-throughput platforms for simultaneous hepatitis virus detection.
Purpose of the Study:
- To develop an integrated automatic electrochemical immunosensor array for simultaneous detection of five hepatitis virus antigens (Hepatitis A, B, C, D, E).
- To establish a rapid, label-free, and cost-effective immunoassay system for multi-analyte detection.
- To evaluate the performance and potential of the developed immunosensor array for point-of-care applications.
Main Methods:
- Immobilization of five hepatitis virus antibodies onto a self-made electrochemical sensor array using nanogold particles and protein A.
- Simultaneous capture of corresponding hepatitis virus antigens from sample solutions in a 1-step format.
- Detection based on potential change measured by a 2-electrode system before and after antigen-antibody reactions.
Main Results:
- Simultaneous determination of five hepatitis virus antigens achieved within 5 minutes.
- Detection limit of less than or equal to 1.0 ng/mL for most analytes.
- High assay precision with intra- and inter-assay coefficients of variation (CVs) less than or equal to 7.3% and 8.1%, respectively.
- Minimal nonspecific adsorption and low crosstalk (< or = 7.5% CV) between sensor sites.
Conclusions:
- The developed electrochemical immunosensor array provides a sensitive, rapid, and cost-effective solution for simultaneous hepatitis virus antigen detection.
- The label-free, multi-analyte immunoassay system demonstrates significant promise for high-throughput diagnostics.
- The chip-based immunosensor array is suitable for miniaturized lab-on-a-chip devices, opening opportunities for point-of-care testing.
