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Label-free fluorescent real-time monitoring of adenylyl cyclase

Hyun-Woo Rhee1, Kyoung-Shim Kim, Pyung-Lim Han

  • 1Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747, Republic of Korea.

Bioorganic & Medicinal Chemistry Letters
|December 22, 2009
PubMed

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...