Sphingopyxis bauzanensis sp. nov., a psychrophilic bacterium isolated from soil

Strain BZ30(T) was isolated from hydrocarbon-contaminated soil. The Gram-negative, aerobic bacterium was psychrophilic and able to grow at temperatures ranging from 1 to 30 °C. The predominant cellular fatty acids of strain BZ30(T) were summed feature 3 (C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH) (37.4 %), C(18 : 1)ω7c (19.6 %), C(16 : 0) (8.2 %), C(14 : 0) 2-OH (8.0 %) and C(16 : 0) 2-OH (5.0 %). The predominant ubiquinone was Q-10. Major polar lipids were sphingoglycolipids, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. Spermidine was the major polyamine. The genomic DNA G+C content was 64.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain BZ30(T) belonged to the family Sphingomonadaceae of the α-4 group of the phylum Proteobacteria, and was related to the members of the genus Sphingopyxis, sharing the highest sequence similarities with the type strains of Sphingopyxis chilensis (98.3 %), S. witflariensis (98.2 %), S. taejonensis (97.4 %) and S. ginsengisoli (97.2 %). On the basis of the phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, strain BZ30(T) represents a novel species of the genus Sphingopyxis, for which the name Sphingopyxis bauzanensis is proposed. The type strain is BZ30(T) (=DSM 22271(T) =CGMCC 1.8959(T) =CIP 110136(T)).