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Direct-imaging-based quantification of Bacillus cereus ATCC 14579 population heterogeneity at a low incubation
Heidy M W den Besten1, Diego Garcia, Roy Moezelaar
1Wageningen University and Research Centre, Laboratory of Food Microbiology, Wageningen, The Netherlands. heidy.denbesten@wur.nl
Applied and Environmental Microbiology
|December 22, 2009
Abstract:
Bacillus cereus ATCC 14579 was cultured in microcolonies on Anopore strips near its minimum growth temperature to directly image and quantify its population heterogeneity at an abusive refrigeration temperature. Eleven percent of the microcolonies failed to grow during low-temperature incubation, and this cold-induced population heterogeneity could be partly attributed to the loss of membrane integrity of individual cells.
