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Related Experiment Video

Updated: Jun 17, 2026

Assaying Blood Cell Populations of the Drosophila melanogaster Larva

Assaying Blood Cell Populations of the Drosophila melanogaster Larva

Published on: November 11, 2015

Hematopoietic stem cells in Drosophila.

Svetlana Minakhina¹, Ruth Steward

¹Rutgers University, Department of Molecular Biology and Biochemistry, Waksman Institute, 190 Frelinghuysen Road, Piscataway, NJ 08854, USA. svetlana@waksman.rutgers.edu

Development (Cambridge, England)

|December 22, 2009

Summary

Drosophila lymph glands harbor hematopoietic stem cells (HSCs) crucial for hemocyte production. The conserved factor Zfrp8/PDCD2 maintains these HSCs, but not their differentiating progeny.

Area of Science:

Developmental biology
Hematopoiesis
Stem cell biology

Background:

The Drosophila lymph gland serves as the primary source of hemocytes, essential for the adult immune system.
Hematopoiesis in Drosophila is regulated by the posterior signaling center (PSC), analogous to vertebrate hematopoietic stem cell niches.

Purpose of the Study:

To identify and characterize hematopoietic stem cells (HSCs) within the Drosophila lymph gland.
To investigate the role of the conserved factor Zfrp8/PDCD2 in HSC maintenance and differentiation.

Main Methods:

Lineage tracing analysis in Drosophila embryos and larvae.
Genetic manipulation to assess the function of Zfrp8/PDCD2.

Main Results:

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Last Updated: Jun 17, 2026

Assaying Blood Cell Populations of the Drosophila melanogaster Larva

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Published on: November 11, 2015

Methods to Examine the Lymph Gland and Hemocytes in Drosophila Larvae

Methods to Examine the Lymph Gland and Hemocytes in Drosophila Larvae

Published on: November 28, 2016

Methods for Imaging Intracellular pH of the Follicle Stem Cell Lineage in Live Drosophila Ovarian Tissue

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Identification of bona fide HSCs in the lymph gland that give rise to a hematopoietic lineage.

Zfrp8/PDCD2 is essential for maintaining HSCs but not for their pluripotent precursor daughter cells.

Pluripotent precursors undergo limited divisions before differentiation.

Conclusions:

HSCs in Drosophila lymph glands are maintained by Zfrp8/PDCD2, highlighting conserved mechanisms in vertebrate hematopoiesis.
Zfrp8/PDCD2 plays a critical, stage-specific role in regulating hematopoietic stem cell populations.