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Branched nanotrees with immobilized acetylcholine esterase for nanobiosensor applications
Klas Risveden1, Kimberly A Dick, Sunil Bhand
1Department of Pure and Applied Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-22100 Lund, Sweden.
Nanotechnology
|December 22, 2009
Summary
Novel nanotree enzyme reactors enhance acetylcholine detection sensitivity. These lab-on-a-chip devices, integrated with regional ion sensitive field effect transistor (RISFET) nanosensors, offer improved performance due to increased surface area for higher enzyme binding capacity.
Area of Science:
- Nanotechnology
- Biosensors
- Enzyme Engineering
Background:
- Development of sensitive biosensors for neurotransmitter detection is crucial.
- Lab-on-a-chip devices offer miniaturization and high throughput.
- Existing nanosensor designs require optimization for enhanced enzymatic activity.
Purpose of the Study:
- To demonstrate a novel lab-on-a-chip nanotree enzyme reactor for acetylcholine detection.
- To compare the enzymatic activity of nanotree reactors with nanorod reactors.
- To investigate the influence of electrical fields on enzyme kinetics in biosensors.
Main Methods:
- Fabrication of gold-tipped branched nanorod (nanotree) and nanorod structures on SiN(x)-covered wafers.
- Enzymatic activity assay using acetylcholine esterase, choline oxidase, and peroxidase.
- Chemiluminescent detection of choline product via luminol reaction.
- Theoretical calculations using quantum chemical methods to assess electrical field effects.
Main Results:
- Nanotree enzyme reactors exhibited significantly higher enzymatic activity compared to nanorod reactors.
- Increased gold surface area of nanotrees likely accounts for higher enzyme binding capacity.
- Theoretical analysis indicated that electrical fields in RISFETs are unlikely to significantly affect acetylcholine esterase kinetics at used strengths.
Conclusions:
- The nanotree enzyme reactor represents a promising advancement for sensitive biosensing applications.
- Optimized nanostructure design can significantly enhance biosensor performance.
- Further research can explore the integration of these reactors into advanced nanosensor systems.
