Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In situ three-dimensional printing for reparative and regenerative therapy.

Biomedical microdevices·2019
Same author

A Perspective on 3D Bioprinting in Tissue Regeneration.

Bio-design and manufacturing·2019
Same author

Sutureless repair of corneal injuries using naturally derived bioadhesive hydrogels.

Science advances·2019
Same author

Hierarchically Patterned Polydopamine-Containing Membranes for Periodontal Tissue Engineering.

ACS nano·2019
Same author

A Microfabricated Sandwiching Assay for Nanoliter and High-Throughput Biomarker Screening.

Small (Weinheim an der Bergstrasse, Germany)·2019
Same author

A simple layer-stacking technique to generate biomolecular and mechanical gradients in photocrosslinkable hydrogels.

Biofabrication·2019

Related Experiment Video

Updated: Jun 17, 2026

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
10:51

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Published on: August 7, 2014

Microscale electroporation: challenges and perspectives for clinical applications.

Won Gu Lee1, Utkan Demirci, Ali Khademhosseini

  • 1Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. termylee@mit.edu

Integrative Biology : Quantitative Biosciences From Nano to Macro
|December 22, 2009
PubMed
Summary

Microscale engineering advances cell research using microscale electroporation for precise control. This technology overcomes conventional limitations, enabling new biological and clinical applications.

More Related Videos

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection
10:34

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Published on: January 7, 2022

Porous Substrate-Based Electroporation with Transepithelial Electrical Impedance Monitoring
08:06

Porous Substrate-Based Electroporation with Transepithelial Electrical Impedance Monitoring

Published on: September 27, 2024

Related Experiment Videos

Last Updated: Jun 17, 2026

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
10:51

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Published on: August 7, 2014

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection
10:34

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Published on: January 7, 2022

Porous Substrate-Based Electroporation with Transepithelial Electrical Impedance Monitoring
08:06

Porous Substrate-Based Electroporation with Transepithelial Electrical Impedance Monitoring

Published on: September 27, 2024

Area of Science:

  • Biotechnology
  • Cell Biology
  • Bioengineering

Background:

  • Microscale engineering miniaturizes devices for in vitro cell research, offering benefits like reduced reagent use and single-cell manipulation.
  • Microscale electroporation provides enhanced spatial and temporal control over electrical parameters, improving upon conventional methods.
  • Conventional electroporation faces limitations including pH variations, electric field distortion, sample contamination, and challenges in cell transfection and viability.

Purpose of the Study:

  • To provide an overview of recent advances in microscale electroporation methods.
  • To discuss the applications of microscale electroporation in biology and its potential for clinical use.
  • To categorize microscale electroporation techniques and examine their suitability for various applications.

Main Methods:

  • Categorization of microscale electroporation into microchannel and microcapillary approaches.
  • Review of recent literature on microscale electroporation techniques and their biological applications.
  • Analysis of clinical applications and challenges for microscale electroporation.

Main Results:

  • Microchannel electroporation enables high-throughput cell transfection under dynamic flow conditions.
  • Microcapillary electroporation allows for controlled cell transfection under static flow conditions.
  • Microscale electroporation shows promise for applications in HIV-1, stem cells, and cancer research.

Conclusions:

  • Microscale electroporation offers significant advantages over conventional methods for cell manipulation.
  • Further advancements are needed to overcome challenges for widespread clinical adoption.
  • Microscale electroporation holds potential for revolutionizing biological research and clinical medicine.