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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Related Experiment Video

Updated: Jun 17, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
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Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

Clickable fluorescent dyes for multimodal bioorthogonal imaging.

Lun K Tsou1, Mingzi M Zhang, Howard C Hang

  • 1The Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, NY 10065, USA.

Organic & Biomolecular Chemistry
|December 22, 2009
PubMed
Summary

Researchers developed new fluorescent dyes for bioorthogonal chemistry, enabling multicolor imaging of biomolecules like proteins. These bright, modular dyes enhance fluorescence imaging capabilities in biological systems.

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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

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Last Updated: Jun 17, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Area of Science:

  • Chemical Biology
  • Organic Synthesis
  • Fluorescence Imaging

Background:

  • Bioorthogonal ligation is crucial for visualizing biomolecules in living systems.
  • Existing fluorescent dyes have limitations in brightness and modularity for bioorthogonal applications.
  • Development of novel, functionalized dyes is needed to advance bioorthogonal fluorescence imaging.

Purpose of the Study:

  • To synthesize novel, clickable fluorescent dyes for bioorthogonal chemistry.
  • To demonstrate the utility of these dyes for multicolor imaging of modified proteins.
  • To explore their application in Förster Resonance Energy Transfer (FRET) studies.

Main Methods:

  • Concise synthesis of fluorescent dyes based on 2-dicyanomethylene-3-cyano-2,5-dihydrofuran scaffolds.
  • Functionalization of dyes with azide and alkyne groups for bioorthogonal ligation.
  • Application of dyes for multicolor imaging of azide- and alkyne-modified proteins.
  • Utilizing dyes in FRET studies to analyze molecular interactions.

Main Results:

  • Successful synthesis of bright and modular azide- and alkyne-functionalized fluorescent dyes.
  • Demonstrated utility for multicolor imaging of modified proteins in biological systems.
  • Successful application in FRET studies, showcasing their versatility.

Conclusions:

  • The developed fluorescent dyes are effective tools for bioorthogonal chemistry.
  • These dyes expand the capabilities of fluorescence imaging in biological research.
  • The modular nature of the dyes allows for broad applicability in various imaging techniques.