Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Source And Potency Of Stem Cells

Source And Potency Of Stem Cells

Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...

Stem Cell Culture

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

Distinctive Features of Adult Stem Cells vs Cancer Stem Cells

Distinctive Features of Adult Stem Cells vs Cancer Stem Cells

A stem cell is an unspecialized cell that can divide without limit as needed and can, under specific conditions, differentiate into specialized cells.
Adult stem cells
Adult stem cells are tissue-specific; hence, they divide to develop the tissue from which they originate. One type of adult stem cell is the epithelial stem cell, which gives rise to the keratinocytes in the multiple layers of epithelial cells in the epidermis of the skin. Adult bone marrow has three distinct types of stem cells:...

Multipotency of Hematopoietic Stem Cells

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Farewell Cytometry Part A.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2024

Same author

Recommendations for using artificial intelligence in clinical flow cytometry.

Cytometry. Part B, Clinical cytometry·2024

Same author

Caffeic Acid Phenethyl Ester (CAPE): Biosynthesis, Derivatives and Formulations with Neuroprotective Activities.

Antioxidants (Basel, Switzerland)·2023

Same author

Counting rare earth metals.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2023

Same author

Bj-PRO-10c, as an allosteric regulator of argininosuccinate synthase, is a potential therapy for neuroblastoma metastasis.

Toxicon : official journal of the International Society on Toxinology·2023

Same author

Purinergic receptors in cognitive disturbances.

Neurobiology of disease·2023

Search research articles

Related Experiment Video

Updated: Jun 17, 2026

Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth

Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth

Published on: November 10, 2023

Phenotypes of stem cells from diverse origin.

Attila Tárnok¹, Henning Ulrich, Jozsef Bocsi

¹Department of Pediatric Cardiology, Heart Centre, University Leipzig, Germany. tarnok@medizin.uni-leipzig.de

Cytometry. Part a : the Journal of the International Society for Analytical Cytology

|December 22, 2009

Summary

Stem cell markers are crucial for identifying various stem cell types, aiding in research and cell therapy. However, marker expression can vary by tissue source and experimental conditions, complicating classification.

More Related Videos

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Related Experiment Videos

Last Updated: Jun 17, 2026

Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth

Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth

Published on: November 10, 2023

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Area of Science:

* Stem cell biology and regenerative medicine.
* Cellular therapy and tissue engineering.

Background:

* Stem cells are vital for development, regeneration, and potential cell therapies.
* They possess self-renewal and multipotency, making them ideal for therapeutic applications.
* Identification and isolation are key for clinical use.

Purpose of the Study:

* To review stem cell marker expression for identifying diverse stem cell lineages.
* To discuss challenges in stem cell identification due to overlapping marker expression.
* To explore how tissue source and experimental conditions affect marker profiles.

Main Methods:

* Comprehensive review of literature on stem cell marker expression.
* Analysis of marker profiles for various stem cell types (embryonic, neural, hematopoietic, mesenchymal, etc.).
* Discussion of advancements in flow cytometry for multi-marker detection.

Main Results:

* No single marker definitively identifies all stem cell types; expression overlaps exist.
* Flow cytometry enables simultaneous detection of multiple markers for improved identification.
* Phenotypic marker differences (e.g., CD34, CD45, CD133) can arise from tissue source or isolation methods.

Conclusions:

* Stem cell identification requires a panel of markers, not a single one.
* Variability in marker expression necessitates careful consideration of cell origin and experimental protocols.
* Further research is needed to standardize stem cell identification for clinical applications.